Nucleic acid synthesis for investigations of gene functions
نویسندگان
چکیده
منابع مشابه
Nucleic Acid Synthesis
INTRODUCTION Synthesis in the field of nucleic acids has been a major and continuing interest in my laboratory ever since we knew the structures of the nucleic acids. Clearly the foundations of synthetic work in the nucleotide field were laid by the prolonged and intensive efforts of Professor Todd's laboratory (Cambridge, England) and it was my good fortune to be associated with this laborator...
متن کاملOrganometallic methodologies for nucleic acid synthesis
A new, general preparation of 2'-5'-linked oligoadenylates has been developed, which relies on: (1) chemoselective 0phosphorylation of N-unblocked nucleosides via hydroxyl activation by Grignard reagents, (2) one-pot construction of internucleotide linkage using bifunctional phosphorylating agents, and (3) selective production of a 3',5'-di-O-protected adenosine. This solution-phase synthesis a...
متن کاملCaffeine-DNA interactions: biochemical investigations comprising DNA-repair enzymes and nucleic acid synthesis.
Chicken embryo cells were treated with caffeine (0.5-8.0 mM) alone or combined with various chemical and physical DNA-and/or chromatin-interactive agents. Analytical procedures comprised scheduled (SDS) and unscheduled (UDS) DNA synthesis, RNA synthesis (RNS), the activities of O6-alkylguanine-DNA alkyltransferase (AT) and poly (ADP-ribose) polymerase (PARP) as well as nucleoid sedimentation. A...
متن کاملNucleic acid carriers for pulmonary gene delivery
The delivery of nucleic acids to the pulmonary route appears to be promising due to non-invasiveness, large area of epithelial surface lining the lung, easy accessibility and ability to provide a platform for local delivery. The therapeutic role of nucleic acids in various diseases pertaining to the pulmonary route e.g. cystic fibrosis, α1-antitrypsin deficiency, asthma, chronic obstructive pul...
متن کاملSensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 1999
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/42.1.17